Laser Focus World Features Alluxa Technology – Fluorescence Imaging: Optical filtering basics for life sciences
Article published in Laser Focus World December 14th 2015
Optical filters can have a dramatic effect on outcomes in life sciences. These principles demonstrate how next-generation thin film enhances excitation and emission in fluorescence bioimaging systems.
Sophisticated optical instrumentation facilitates advanced bioimaging, and high-performance optical filtering improves fluorescence detection systems. Because proper optical filtering boosts throughput and enables wide-scale blocking, it solves problems like increasing the sensitivity of a system to detect infinitesimally small fluorescent signals emitted from biological samples. Too much light can lead to overexposure at the sensor, thus masking the signal—the filter helps to remove unwanted light so that the fluorescence response can be identified and measured. In fact, filters are arguably the most important element defining system performance for fluorescence detection. And thanks to manufacturing innovation, they can do so at competitive price points. Let’s take a look at some important concepts in optical filtering and how they can improve outcomes in biomedical research.